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Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
 

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

Article DOI: 10.3791/55357-v • 06:19 min • July 18th, 2017
July 18th, 2017 •
1, 1, 1, 1, 1,2,3, 1, 1,2,3
1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 2German Cancer Consortium (DKTK), 3German Cancer Research Center (DKFZ)

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We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.

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Keywords: Circulating Tumor Cells Orthotopic Mouse Model Colorectal Cancer Microsurgical Technique Cecal Wall Injection Reproducible Metastatic Tumors Immunocompromised Mouse Intra-cecal Injection Micro Injection Pump Binocular Surgical Microscope Density Gradient Medium Blood Sample Collection
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