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Rekombinant α-β - och γ-Synucleins stimulera Protein 2A katalytisk subenhet fosfatasaktiviteten Cell gratis analyser
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Capítulos
- 00:00Título
- 00:33Preparation of Buffers and Reagents
- 00:38Preparing p-Nitrophenol Phosphate (pNPP) Buffer
- 01:21Preparing Malachite Green Solution
- 02:21Preparation of Activator
- 02:42Preparing Malachite Green + Tween 20 (MGT) Working Solution
- 02:58Preparing Threonine Phosphopeptide (pT)
- 03:31Synuclein Preparation
- 03:59Preparing Phosphate Standards
- 04:45Phosphate Standards Step 2
- 05:17PP2A Activity Assay
- 06:42Photometric Analysis
- 06:53Representative Results
- 09:01Conclusion
Denna skrift presenterar ett protokoll som visar hur man mäter aktiviteten av rekombinant protein fosfatas 2A katalytisk subenhet (PP2Ac) som svar på rekombinant α-synuclein, β-synuclein eller γ-synuclein proteiner med en enkel kolorimetriska analys. Vi visar slutsatser angående α-synuclein specificitet mot PP2Ac i mus hjärnan.
