Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Recombinant α- β- and γ-Synucleins …
JoVE Journal
Neuroscience
Author Produced

This content is Open Access.

JoVE Journal Neuroscience
Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
 

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Article DOI: 10.3791/55361-v • 09:36 min • August 13th, 2017
August 13th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Biomedical Sciences, Center of Emphasis in Neurosciences, Graduate School of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso

Chapters

Summary
Automatic Translation

This publication presents a protocol showing how to measure the activity of recombinant protein phosphatase 2A catalytic subunit (PP2Ac) in response to recombinant α-synuclein, β-synuclein, or γ-synuclein proteins using a simple colorimetric assay. We show findings regarding α-synuclein specificity toward PP2Ac in mouse brain.

Tags

Keywords: Recombinant Synuclein Protein Phosphatase 2A Cell-free Assay P-Nitrophenol Phosphate (pNPP) Buffer Malachite Green Solution Tween 20 Activator Threonine Phosphopeptide Phosphate Standards
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter