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Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
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Neurociência
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JoVE Journal Neurociência
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

DOI:
10.3791/55397-v

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11:55 min

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April 03, 2017

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1Centre de recherche de l’Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience,Université Laval, 2Centre de recherche du CHU Sainte-Justine

Capítulos

  • 00:05Título
  • 00:43Pre-embedding Immunohistochemistry
  • 03:11Osmification and Embedding for Electron Microscopy Observation
  • 06:23Sample Preparation for Ultrathin Sectioning and Observation Using a Transmission Electron Microscope
  • 09:05Results: Immunolabeling of the Squirrel Monkey Globus Pallidus for ChAT, SERT and TH
  • 11:08Conclusion

Summary

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Here, we provide an easy, low-cost, and time-efficient protocol to chemically fix primate brain tissue with acrolein fixative, allowing for long-term preservation that is compatible with pre-embedding immunohistochemistry for transmission electron microscopy.

Tags

Non-human PrimatePre-embedding ImmunohistochemistryElectron MicroscopyAcrolein FixationSodium BorohydrideNormal SerumCold Fish GelatinPrimary AntibodySecondary AntibodyAvidin-biotin-peroxidase33′-DiaminobenzidineHydrogen PeroxideOsmium Tetroxide

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