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非人类灵长类动物脑组织的制备预嵌入免疫组化和电子显微镜

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

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非人类灵长类动物脑组织的制备预嵌入免疫组化和电子显微镜

DOI:

10.3791/55397-v

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11:55 min

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April 03, 2017

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Lara Eid², Martin Parent

¹Centre de recherche de l’Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience,Université Laval, ²Centre de recherche du CHU Sainte-Justine

Capítulos

00:05Título
00:43Pre-embedding Immunohistochemistry
03:11Osmification and Embedding for Electron Microscopy Observation
06:23Sample Preparation for Ultrathin Sectioning and Observation Using a Transmission Electron Microscope
09:05Results: Immunolabeling of the Squirrel Monkey Globus Pallidus for ChAT, SERT and TH
11:08Conclusion

Summary

Tadução automática

在这里，我们提供了一种简单，低成本，和时间高效的协议进行化学固定与丙烯醛固定液灵长类脑组织，从而允许长期保存，其与预嵌入-免疫组化透射电子显微镜兼容。

Tags

Non-human Primate Pre-embedding Immunohistochemistry Electron Microscopy Acrolein Fixation Sodium Borohydride Normal Serum Cold Fish Gelatin Primary Antibody Secondary Antibody Avidin-biotin-peroxidase 3 3′-Diaminobenzidine Hydrogen Peroxide Osmium Tetroxide

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