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Udarbejdelse af ikke-menneskelig primat Brain Tissue til Pre-indlejring Immunohistokemi og Electron Microscopy
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JoVE Journal Neurociência
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

Udarbejdelse af ikke-menneskelig primat Brain Tissue til Pre-indlejring Immunohistokemi og Electron Microscopy

DOI:
10.3791/55397-v

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11:55 min

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April 03, 2017

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1Centre de recherche de l’Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience,Université Laval, 2Centre de recherche du CHU Sainte-Justine

Capítulos

  • 00:05Título
  • 00:43Pre-embedding Immunohistochemistry
  • 03:11Osmification and Embedding for Electron Microscopy Observation
  • 06:23Sample Preparation for Ultrathin Sectioning and Observation Using a Transmission Electron Microscope
  • 09:05Results: Immunolabeling of the Squirrel Monkey Globus Pallidus for ChAT, SERT and TH
  • 11:08Conclusion

Summary

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Tadução automática

Her, giver vi en let, billig og tidsbesparende protokol til kemisk fix primat hjernevæv med acrolein fiksativ, giver mulighed for langsigtet opbevaring, der er kompatibel med præ-embedding immunhistokemi transmissionselektronmikroskopi.

Tags

Keywords: Non-human PrimatePre-embedding ImmunohistochemistryElectron MicroscopyAcrolein FixationSodium BorohydrideNormal SerumCold Fish GelatinPrimary AntibodySecondary AntibodyAvidin-biotin-peroxidase33′-DiaminobenzidineHydrogen PeroxideOsmium Tetroxide

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