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JoVE Journal Biochemistry
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
 

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Article DOI: 10.3791/55406-v • 09:06 min • May 1st, 2017
May 1st, 2017 •
1, 1, 2, 3, 1
1Department of Chemistry, University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science, Janssen Research and Development

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Combined precursor isotopic labeling and isobaric tagging (cPILOT) is a quantitative proteomics strategy that enhances sample multiplexing capabilities of isobaric tags. This protocol describes the application of cPILOT to tissues from an Alzheimer's disease mouse model and wild-type controls.

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Keywords: Enhanced Sample Multiplexing Combined Precursor Isotopic Labeling Isobaric Tagging CPILOT Alzheimer's Disease Mouse Model Tissue Samples Protein Changes Aging Neurodegenerative Disease Cancer Pathogenesis Diagnosis Prognosis Therapeutic Targets Acetylation Protein Nitration Brain Heart Liver Tissue Homogenization BCA Assay Protein Concentration Dithiothreitol Iodoacetamide L-cysteine Trypsin Digestion
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