The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Maryl&#232;ne Vandevenne; Mathieu Dondelinger; Sami Yunus; Astrid Freischels; R&#233;gine Freischels; Oscar Crasson; Noureddine Rhazi; Pierre Bogaerts; Moreno Galleni; Patrice Fil&#233;e

doi:10.3791/55414

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Bioengenharia

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Brug af en β-lactamase-baseret Conductimetric Biosensor Assay til påvisning Biomolekylær interaktioner

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Bioengenharia

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JoVE Journal Bioengenharia

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Brug af en β-lactamase-baseret Conductimetric Biosensor Assay til påvisning Biomolekylær interaktioner

DOI:

10.3791/55414-v

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08:06 min

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February 01, 2018

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Marylène Vandevenne*¹, Mathieu Dondelinger*¹, Sami Yunus, Astrid Freischels, Régine Freischels, Oscar Crasson, Noureddine Rhazi, Pierre Bogaerts, Moreno Galleni, Patrice Filée

¹Life Science Department,University of Liège, ²Institute of Condensed Matter and Nanoscience,Catholic University of Louvain, ³Laboratory of Clinical Microbiology,Catholic University of Louvain, ⁴Biotechnology Department,CER Group

Capítulos

00:05Título
00:52Sensor Preparation and Regeneration
02:04Binding Assay Performed on the Sensor
04:45Results: Measurement of Conducticity Cganges Upon Antibiotic Hydrolysis by the Immobilization Chimeric β-Lactamase
06:35Conclusion

Summary

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I dette arbejde rapportere vi en ny metode til at undersøge protein-protein interaktioner ved hjælp af en conductimetric biosensor baseret på β-lactamase Hybridteknologien. Denne metode bygger på frigivelse af protoner ved hydrolyse af β-lactamer.

Brug af en β-lactamase-baseret Conductimetric Biosensor Assay til påvisning Biomolekylær interaktioner

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