Journal
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Immunology and Infection
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Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

DOI:
10.3791/50719-v

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11:56 min

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October 25, 2013

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, ,
1London Centre for Nanotechnology and Departments of Medicine,University College London

Chapters

  • 00:05Title
  • 02:21Preparation and Functionalization of Cantilevers
  • 06:03Test Drug Preparations
  • 07:24Surface Stress Detection
  • 10:05Results: Quantification of Changes in Drug-target Binding Interactions
  • 11:30Conclusion

Summary

Automatic Translation

Automatic Translation

Acquired resistance to antibiotics is a major public healthcare problem and is presently ranked by the WHO as one of the greatest threats to human life. Here we describe the use of cantilever technology to quantify antibacterial resistance, critical to the discovery of novel and powerful agents against multidrug resistant bacteria.

Tags

Keywords: NanomechanicsDrug-target InteractionsAntibiotic ResistanceCantilever SensorBacterial Cell WallVancomycinLangmuir AdsorptionThermodynamic Equilibrium Dissociation ConstantPeptide Receptor DistributionAntibacterial Sensing

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