Journal
/
Immunology and Infection
/
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

DOI:
10.3791/51400-v

•

05:46 min

•

April 09, 2014

•

,
1HIV Drug Resistance Program,National Cancer Institute

Chapters

  • 00:05Title
  • 01:09Viral Stock Generation
  • 02:29Compound Screening
  • 03:42Measurement of Luciferase Activity
  • 04:41Results: Representative Luciferase Signal Readout Inhibition
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe cellular cytotoxicity and single round infectivity assays that allow for the rapid and accurate screening of compounds to determine their cellular cytotoxicity (CC50) and IC50 values against WT and drug resistant HIV-1.

Tags

HIV Reverse TranscriptaseHIV IntegraseAntiviral CompoundsDrug ResistanceCytotoxicity AssayLuciferase ReporterViral InfectivityScreening PlatformHigh-throughput Screening

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

In vitro Uncoating of HIV-1 Cores
check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
check_url/51400?article_type=v&slug=rapid-screening-of-hiv-reverse-transcriptase-and-integrase-inhibitors

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Read Article