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Immunology and Infection

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JoVE Journal Immunology and Infection
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
 

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Article DOI: 10.3791/51222-v • 11:34 min • May 5th, 2014
May 5th, 2014 •
1, 2, 2, 1, 3, 1, 1
1Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur

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Summary
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In vitro assays to measure virus replication have been greatly improved by the development of recombinant RNA viruses expressing luciferase or other enzymes capable of bioluminescence. Here we detail a high-throughput screening pipeline that combines such recombinant strains of measles and chikungunya viruses to isolate broad-spectrum antivirals from chemical libraries.

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Keywords: High-throughput Screening Broad-spectrum Chemical Inhibitors RNA Viruses Cellular Pathways Host Cell Functions Antiviral Compounds Recombinant Viruses Reporter Enzymes Bioluminescence Luciferase Measles Virus Chikungunya Virus Cell Culture Assays
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