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A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
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Chapters
- 00:04Introduction
- 01:06Create Linker‐Coupled Antigen Solution
- 02:36Preparation of Antigen Labeled Serum and the 6‐Plex Plate
- 03:54Serum/Antigen Incubates Transfer into the 6‐Plex Plate
- 04:42Washing the 6‐Plex Plate and Reading Buffer Addition
- 05:11Results: Comparison of Antibody Levels Study Between the Single and the 6‐Plex ECL Assay
- 05:59Conclusion
In line with the urgent need for screening for type 1 diabetes, celiac disease, and coronavirus disease 2019, we developed a high throughput 6-Plex electrochemiluminescence assay to simultaneously detect all four islet autoantibodies, tissue transglutaminase autoantibodies, and antibodies to the receptor binding domain of severe acute respiratory syndrome coronavirus 2.
