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Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy
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JoVE Journal Medicina
Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

DOI:
10.3791/55450-v

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06:49 min

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May 31, 2017

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, , ,
1Department of Translational Skin Cancer Research,University of Duisburg/Essen, 2Institute for Experimental Immunology and Imaging,University of Duisburg/Essen, 3Imaging Center Essen, Electron Microscopy Unit,University Hospital of Essen

Capítulos

  • 00:05Título
  • 00:56Diptheria Toxin (DPT) Myocarditis Induction and Light-sheet Microscope Sample Preparation
  • 02:12Light-sheet Microscopy and Image Post-processing
  • 05:16Results: Representative 3D Recordings of Whole Organ Light-sheet Microscopy
  • 05:58Conclusion

Summary

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Tadução automática

Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45+ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.

Tags

Leukocyte InfiltrateMurine ModelFulminant MyocarditisLight Sheet MicroscopyWhole Organ VisualizationCardiac ArrhythmiaCellular ResolutionInflammation QuantificationCardiac ProsthesisFluorescence MicroscopyImage Processing

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