É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery,Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences,Johns Hopkins University School of Medicine
Capítulos
- 00:05Título
- 00:37Expression of Recombinant HA-MOR1, Cell Fractionation, and Membrane Collection
- 03:55[35S]GTPγS Binding
- 06:05Membrane Filtration
- 06:59Liquid Scintillation Counting
- 07:57Results: Characterization of G-protein-coupled Receptors by Monitoring Radio-labled GTP Binding
- 09:01Conclusion
Guanosine triphosphate (GTP) מחייב הוא אחד האירועים המוקדמים G-Protein-Coupled קולטן (GPCR) ההפעלה. פרוטוקול זה מתאר כיצד לאפיין באופן פרמקולוגי אינטראקציות ספציפיות GPCR-ligand על ידי ניטור מחייב של אנלוגי GTP, שכותרתו רדיו, [ 35 S] guanosine-5'-O- (3-thio) triphosphate ([ 35 S] GTPγS), in תגובה ליגנד של עניין.
