Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Muhammad Khairul Ramlee; Jing Wang; Alice M. S. Cheung; Shang Li

doi:10.3791/55586

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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

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Genética

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JoVE Journal Genética

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

DOI:

10.3791/55586-v

•

08:25 min

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April 08, 2017

•

Muhammad Khairul Ramlee, Jing Wang, Alice M. S. Cheung, Shang Li

¹Cancer & Stem Cell Biology Programme,Duke-NUS Medical School

Capítulos

00:05Título
00:55Sample Preparation and Amplification of CRISPER/Cas9 Regions with Fluorescent PCR
04:32Capillary Gel Electrophoresis and Electropherogram Analysis
06:25Results: Genotyping HEPG2 Targeted with CRISPER/Cas9 Construct Against NAP1L1
07:27Conclusion

Summary

Tadução automática

The genotyping technique described here, which couples fluorescent polymerase chain reaction (PCR) to capillary gel electrophoresis, allows for high-throughput genotyping of nuclease-mediated knockout clones. It circumvents limitations faced by other genotyping techniques and is more cost effective than sequencing methods.

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

•

•

•

Capítulos

Summary

Tadução automática

Tags

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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