/

/

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

DOI:

10.3791/55653-v

•

08:12 min

•

July 11, 2017

•

Tanumoy Saha, Isabel Rathmann, Milos Galic

¹DFG Cluster of Excellence ‘Cells in Motion’, (EXC 1003), Institute of Medical Physics and Biophysics,University of Muenster

Capítulos

00:05Título
00:43Cell Culture
01:42Image Acquisition and Analysis
06:54Results: Filopodia Analysis in COS Cells Transfected with f-actin
07:37Conclusion

Summary

Tadução automática

We present a software solution for semi-automated tracking of relative protein concentration along the length of dynamic cellular protrusions.

Tags

Protein Concentration Dynamic Cellular Protrusions Graphical User Interface Software-assisted Tracking Filopodial Dynamics Spatially Resolved Protein Localization Adaptive Shape Tracking Algorithm Cell Biology Filopodia Extension And Retraction Transfected Cells Image Acquisition Signal-to-noise Ratio Microscopy Image Pre-processing Graphical User Interface

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Read Article