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JoVE Journal Neuroscience
Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice
 

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

Article DOI: 10.3791/55770-v • 08:32 min • July 13th, 2017
July 13th, 2017 •
1, 1, 1, 1, 2, 2, 1, 1, 3, 3, 3, 4, 4, 1,4
1Neuroregeneration Laboratory, Department of Anesthesiology, University of California, San Diego, 2Institute of Animal Physiology and Genetics, Czech Academy of Sciences, 3Department of Neurosurgery, University of California, San Diego, 4Institute of Neurobiology, Slovak Academy of Sciences

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Resumo
Automatic Translation

The goal of the present study was to develop and validate the potency and safety of spinal adeno-associated virus 9 (AAV9)-mediated gene delivery by using a novel subpial gene delivery technique in adult mice.

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Keywords: Subpial AAV9 Vector Delivery Spinal Cord Spinal Gene Expression Spinal Neurodegenerative Disorders Amyotrophic Lateral Sclerosis Spinal Traumatic Injury Chronic Pain Transgene Expression Spinal Gray Matter Spinal White Matter Spinal Segments Dura-opening Subpial Needle Anesthesia Isoflurane Paravertebral Muscle Spinal Laminectomy Dura Incision Cervical Occipital Bone
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