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通过流式细胞术分析来自中枢神经系统的小胶质细胞和单核细胞衍生的巨噬细胞
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
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通过流式细胞术分析来自中枢神经系统的小胶质细胞和单核细胞衍生的巨噬细胞

DOI:
10.3791/55781-v

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10:43 min

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June 22, 2017

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, , ,
1Inserm U 1227, CNRS UMR 7225, 2Sorbonne Universités,UPMC, University of Paris, 3Institut du Cerveau et de la Moelle épinière, ICM, 4AP-HP, Hôpital de la Pitié Salpêtrière;

Capítulos

  • 00:05Título
  • 00:52Perfusion and Brain and Spinal Cord Dissection
  • 03:18Cell Dissociation and Density Gradient Separation
  • 06:00Cell Labeling for Flow Cytometry
  • 08:06Results: Representative Brain and Spinal Cord Macrophage Subpopulation Analysis
  • 09:31Conclusion

Summary

Tadução automática

Tadução automática

该方案通过流式细胞术提供成年小鼠中枢神经系统中巨噬细胞亚群的分析，有助于研究由这些细胞表达的多种标志物。

Tags

MicrogliaMonocyte-derived MacrophagesCentral Nervous SystemFlow CytometryNeuroimmunologyMacrophage SubpopulationsCell Marker AnalysisCell IsolationSpinal CordBrain

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