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Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
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Bioquímica
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JoVE Journal Bioquímica
Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

DOI:
10.3791/55790-v

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11:20 min

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January 24, 2018

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1Laboratory of Molecular Biology,Medical Research Council

Capítulos

  • 00:05Título
  • 00:59System 1: Preparation of 72 Crystallization Plates Filed with Screening Kits
  • 04:46System 2: Setup of 100 nL Crystallization Droplets from a Single Sample in 20 96-well Plates
  • 09:22Results: Initial Screening of Outer Membrane Protein F (OmpF) and MreB Crystallization Conditions
  • 09:48Conclusion

Summary

Tadução automática

Tadução automática

Automated systems and protocols for the routine preparation of a large number of screens and nanoliter crystallization droplets for vapor diffusion experiments are described and discussed.

Tags

Macromolecular CrystallizationProtein StructureDNARNAVapor DiffusionNanoliter DispenserInkjet PrinterAdhesive Plate SealerLiquid HandlerChillerCarouselScreening Kits96-well Layout

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