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Study of Protein-protein Interactions in Autophagy Research
 

Study of Protein-protein Interactions in Autophagy Research

Article DOI: 10.3791/55881-v • 14:09 min • September 9th, 2017
September 9th, 2017 •
1, 1, 2, 1,3
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Information Center, Sabanci University, 3EFSUN, Center of Excellence for Functional Surfaces and Interfaces for Nano Diagnostics, Sabanci University

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Presented here are two antibody-based protein-protein interaction research techniques: immunofluorescence and immunoprecipitation. These techniques are suitable for studying physical interactions between proteins for the discovery of novel components of cellular signaling pathways and for understanding protein dynamics.

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Keywords: Protein-protein Interactions Autophagy Molecular Biology Biochemistry Cell Biology Immunoprecipitation Immunofluorescence Confocal Microscopy Protein Localization Cell Culture Fixation PFA
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