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Immunology and Infection

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JoVE Journal Immunology and Infection
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
 

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Article DOI: 10.3791/55997-v • 08:43 min • June 24th, 2017
June 24th, 2017 •
1, 1, 1, 2, 2, 1
1Laboratory of Cellular & Molecular Biology, National Cancer Institute, National Institutes of Health, 2Laboratory of Single Molecule Biophysics, National Heart, Lung, and Blood, Institute, National Institutes of Health

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We demonstrate a method to image multiple molecules within heterogeneous nano-structures at single molecule accuracy using sequential binding and elution of fluorescently labeled antibodies.

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Multiplexed Super-resolution Imaging T Cells MadSTORM Single Molecule Localisation Microscopy Proteins T Cell Activation Distribution Microcluster Components Greater Multiplexing Supervised Solution Microscopy Methods Antibodies Alexa 647 Dye Antibody Labeling Kit Centrifugation Aggregated Antibodies A12 Cover Slip Chambers Poly-l-lyzine Dilution Fluorescent Nanodiamonds (FNDs) PBS Vortex Sonicate Incubate Washing
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