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Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
 

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Article DOI: 10.3791/56001-v • 11:29 min • July 10th, 2017
July 10th, 2017 •
1,2, 2, 2,3, 2,4, 2,3
1Departments of Pathology and Infectious Diseases and Microbiology, University of Pittsburgh, 2University of Pittsburgh Cancer Institute, 3Department of Environmental and Occupational Health, University of Pittsburgh, 4Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences, University of Pittsburgh

Capítulos

Resumo
Automatic Translation

In this article, a detailed protocol for quantifying telomere length using a modified terminal restriction fragment analysis is discussed that provides fast and efficient direct measurement of telomere length. This technique can be applied to a variety of cell sources of DNA for quantifying telomere length.

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Modified Terminal Restriction Fragment Analysis Quantifying Telomere Length In-gel Hybridization Telomere Restriction Fragment Analysis Procedure Telomeres In Eukaryotic Cells Telomere Length Measurement Kilobases Genomic DNA Samples DNA Extraction Kit DNA Digestion Rsa1 Restriction Enzyme Hinf1 Restriction Enzyme Agarose Solution Gel Electrophoresis
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