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大鼠电癫痫发作与海马的分离检测突触后密度蛋白的变化

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

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大鼠电癫痫发作与海马的分离检测突触后密度蛋白的变化

DOI:

10.3791/56016-v

•

09:07 min

•

August 15, 2017

•

Sung-Soo Jang², Han Gil Jeong, Hee Jung Chung²

¹Department of Molecular and Integrative Physiology,University of Illinois at Urbana-Champaign, ²Neuroscience Program,University of Illinois at Urbana-Champaign

Capítulos

00:05Título
01:08Preparation of an ECS Machine
01:47Induction of Acute ECS
03:07Homogenization and Fractionation of Rat Hippocampi
05:20Isolation of the PSD from the Crude Membrane Protein Fraction
06:32Results: Examination of Synaptic Proteins in Hippocampal S2, P2, and PSD Fractions from Rats that Received NS or Acute ECS
07:55Conclusion

Summary

Tadução automática

电癫痫 (ECS) 是一种电治疗严重抑郁症的实验动物模型。ECS 全球刺激海马活动, 导致突和突触可塑性。在这里, 我们描述的方法的 ECS 诱导大鼠和亚细胞分型的海马, 以检查癫痫诱导的变化, 突触蛋白。

Tags

Keywords: Electroconvulsive Seizure Hippocampus Postsynaptic Density Proteins Neuroplasticity Pulse Generator Seizure Induction Racine Scale Hippocampal Fractionation

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