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JoVE Journal Developmental Biology
The Aortic Ring Co-culture Assay
 

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

Article DOI: 10.3791/56083-v • 10:04 min • September 18th, 2017
September 18th, 2017 •
1,2, 1, 1,2, 1,2,3,4,5
1Create Fertility Centre, 2Department of Physiology, University of Toronto, 3Department of Obstetrics and Gynecology, University of Toronto, 4Department of Medical Sciences, University of Toronto, 5Department of Obstetrics and Gynecology, Women's College Hospital

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Resumo
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Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.

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Keywords: Aortic Ring Co-culture Assay Angiogenic Potential Mesenchymal Stromal Cells Regenerative Therapy Vascular Targeting Ischemic Diseases Functional Assay In Vitro Basal Membrane Extract (BME) Tissue Culture Aorta Isolation Intercostal Arteries Aortic Rings Embedding
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