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传染性噬菌体大肠杆菌-合成基于无细胞表达系统

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Bioengenharia

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JoVE Journal Bioengenharia

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

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传染性噬菌体大肠杆菌-合成基于无细胞表达系统

DOI:

10.3791/56144-v

•

11:33 min

•

August 17, 2017

•

Mark Rustad, Allen Eastlund, Ryan Marshall, Paul Jardine, Vincent Noireaux

¹School of Physics and Astronomy,University of Minnesota, ²Department of Diagnostic and Biological Sciences and Institute for Molecular Virology,University of Minnesota

Capítulos

00:05Título
00:37Phage Amplification
02:15Purification of Phage
05:39Double-stranded Genome DNA Extraction
08:19Cell-free Phage Reaction and Phage Titer Experiment
09:35Results: Synthesis and Quantification of Phage T7 in Varying Molecular-crowding Environments
10:46Conclusion

Summary

Tadução automática

新一代的无细胞的转录翻译平台被设计在生化系统体外基因电路执行构建。在这篇文章中，我们描述如何从其基因组，使用所有的大肠杆菌细胞免费 TXTL 系统合成噬菌体 MS2、 ΦΧ174 和 T7，等。

Tags

Bacteriophage Synthesis E. Coli Cell-free Expression System Phage Plaques Phage Infection DNase Sucrose Gradient Ultracentrifugation Phage Band Extraction

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