É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:15Introduction
- 01:06Tools and Reagents
- 03:13Mounting Brain onto Vibraslicer
- 04:26Removing and Sectioning Brain
- 08:12Isolating and Processing Cortex
- 11:29Washing Cortical Pieces
- 13:14Trituration of Cortical Pieces
- 16:35Visualizing Cortical Neurons
- 17:12Conclusion
This video shows a procedure for generating neuronal cultures from late embryo and early postnatal mouse cortex. These cultures can be used for immunocytochemistry, biochemistry, electrophysiology, calcium and sodium imaging and provide a platform to study the neuronal development of transgenic animals that carry a postnatal lethal gene mutation.
Tags
Dissociated Mouse Cortical Neuron CulturesLate EmbryoEarly PostnatalMouse BrainImmunocytochemistryBioquímicaElectrophysiologyCalcium ImagingSodium ImagingProtein IsolationRNA IsolationTransgenic AnimalsGene MutationCortical RindThalamo-cortical Fiber TractNon-neuronal CellsNeuronal CellsEnzyme Incubation StepGlia Feeder Cells
