Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

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Genética

JoVE Journal
Genética

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JoVE Journal Genética

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

DOI:

10.3791/56313-v

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09:08 min

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November 13, 2017

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Ivana Grbesa, Miriam Tannenbaum, Avital Sarusi-Portuguez, Michal Schwartz, Ofir Hakim

¹The Mina and Everard Goodman Faculty of Life Sciences,Bar-Ilan University

Capítulos

00:05Título
00:54Purification of Naïve Human CD4+ Cells, Polarization to Th1 and TH2 Cells, and Nuclei Extraction
03:18Transposition Reaction and Library Amplification
04:33Size Selection of ATAC-seq Libraries
06:48Results: Quality Control and Next Generation Sequencing Reads
08:07Conclusion

Summary

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Assay for Transposase-Accessible Chromatin coupled with high-throughput sequencing (ATAC-seq) is a genome-wide method to uncover accessible chromatin. This is a step-by-step ATAC-seq protocol, from molecular to the final computational analysis, optimized for human lymphocytes (Th1/Th2). This protocol can be adopted by researchers without prior experience in next-generation sequencing methods.

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

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