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ATAC 基因组在原发性人类 T 淋巴细胞中的染色体全易接近染色质

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Genética

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JoVE Journal Genética

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

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ATAC 基因组在原发性人类 T 淋巴细胞中的染色体全易接近染色质

DOI:

10.3791/56313-v

•

09:08 min

•

November 13, 2017

•

Ivana Grbesa, Miriam Tannenbaum, Avital Sarusi-Portuguez, Michal Schwartz, Ofir Hakim

¹The Mina and Everard Goodman Faculty of Life Sciences,Bar-Ilan University

Capítulos

00:05Título
00:54Purification of Naïve Human CD4+ Cells, Polarization to Th1 and TH2 Cells, and Nuclei Extraction
03:18Transposition Reaction and Library Amplification
04:33Size Selection of ATAC-seq Libraries
06:48Results: Quality Control and Next Generation Sequencing Reads
08:07Conclusion

Summary

Tadução automática

转-可获得的染色质结合高通量测序 (ATAC-seq) 是一个基因组范围的方法, 以揭示可获得的染色质。这是一个 step-by 步骤的 ATAC-seq 协议, 从分子到最终的计算分析, 优化为人类淋巴细胞 (Th1/Th2)。这一协议可以被研究人员采用, 而无需事先经验的下一代测序方法。

Tags

Keywords: ATAC-Seq Chromatin Accessibility Genome-wide Regulatory Elements T Lymphocytes Epigenomics Primary Cells Transposase Nuclei Isolation Th1 Th2 Cell Activation

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