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Biologia
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Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney
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Biologia
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JoVE Journal Biologia
Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

DOI:
10.3791/56324-v

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08:26 min

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January 08, 2018

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, , , , ,
1Department of Veterans Affairs,Tennessee Valley Healthcare System, 2Departments of Medicine and Pharmacology,Vanderbilt University Medical Center, 3Department of Medicine,Baylor University College of Medicine

Capítulos

  • 00:05Título
  • 00:52Preparation for Surgery
  • 01:39Injection Surgery
  • 04:03Injection Efficiency and Transgene Effects Assessment
  • 06:06Results: Hydrodynamic Renal Pelvis Injection Results in Kidney-specific Gene Expression
  • 07:25Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes a method to inject plasmid DNA into the mouse kidney via the renal pelvis to produce transgene expression specifically in the kidney.

Tags

Hydrodynamic Renal Pelvis InjectionProtein ExpressionPlasmid DNAKidneyNephrologyVisual DemonstrationSurgical ProcedureMouseAnesthesiaIncisionKidney ExposurePlasmid DNA Injection

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