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Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
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JoVE Journal Pesquisa do Câncer
Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

DOI:
10.3791/56332-v

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07:44 min

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March 12, 2018

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,
1Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health, 2BC Cancer Agency,Provincial Health Services Authority, 3Department of Pathology and Laboratory Medicine,University of British Columbia

Capítulos

  • 00:04Título
  • 00:52Tail Vein Injection and Lung Insufflation
  • 02:48Widefield Fluorescence Lung Slice Imaging and Analysis
  • 05:49Results: Representative Lung Slice Imaging Analysis
  • 06:44Conclusion

Summary

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The goal of this article is to provide a detailed description of the protocol for the pulmonary metastasis assay (PuMA). This model permits researchers to study metastatic osteosarcoma (OS) cell growth in lung tissue using a widefield fluorescence or confocal laser-scanning microscope.

Tags

Metastatic Lung ColonizationOsteosarcomaPulmonary Metastasis AssayTrachea CannulationAgarose InsufflationThree-dimensional MicroenvironmentTumor Cell VisualizationLung SlicesEx Vivo Culture

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