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Op lange termijn levende Imaging Device voor verbeterde experimentele manipulatie van zebravis larven
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1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Morgridge Institute for Research,University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation,University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program,University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology,University of Wisconsin-Madison, 6Department of Pediatrics,University of Wisconsin-Madison
Capítulos
- 00:05Título
- 00:57Design and 3D Printing of Molds
- 02:57PDMS Fabrication of zWEDGI Device
- 04:43Plasma-bonding zWEDGI to Glass Dish
- 07:19Channel Preparation and Loading Larvae
- 09:52Wounding and Imaging Larvae and End of Experiment
- 11:14Results: Post Wound Live Imaging and 3D Reconstruction of Zebrafish Larvae Tail Fin
- 12:36Conclusion
Dit manuscript beschrijft de zWEDGI (zebrafish Wounding en Entrapment apparaat voor groei en Imaging), die is een verzuilde apparaat ontworpen bedwingen zebrafish larven te oriënteren. Het ontwerp staat staart transect en op lange termijn collectie van hoge-resolutie fluorescentie microscopie beelden van wondgenezing en regeneratie.
