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Langfristige Live Imaging-Gerät für verbesserte experimentelle Manipulation von Zebrafischlarven
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1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Morgridge Institute for Research,University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation,University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program,University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology,University of Wisconsin-Madison, 6Department of Pediatrics,University of Wisconsin-Madison
Capítulos
- 00:05Título
- 00:57Design and 3D Printing of Molds
- 02:57PDMS Fabrication of zWEDGI Device
- 04:43Plasma-bonding zWEDGI to Glass Dish
- 07:19Channel Preparation and Loading Larvae
- 09:52Wounding and Imaging Larvae and End of Experiment
- 11:14Results: Post Wound Live Imaging and 3D Reconstruction of Zebrafish Larvae Tail Fin
- 12:36Conclusion
Dieses Manuskript beschreibt die zWEDGI (Zebrafisch daraus und verlockende Falle Gerät für Wachstum und Imaging), die ist ein unterteilte Gerät zu orientieren und zurückhalten Zebrafischlarven. Das Design erlaubt Schweif Durchtrennung und langfristige Sammlung von hochauflösender Fluoreszenz-Mikroskopie-Bildern der Wundheilung und Regeneration.
