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JoVE Journal Bioengineering
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
 

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Article DOI: 10.3791/56585-v • 17:17 min • June 3rd, 2018
June 3rd, 2018 •
1, 2,3, 1, 4
1Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 2Laboratory of Functional Device, Institute of Advanced Technology, Universiti Putra Malaysia, 3Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 4La Trobe Institute for Molecular Science, La Trobe University

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Resumo
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A protocol for the development of an electrochemical DNA biosensor comprising a polylactic acid-stabilized, gold nanoparticles-modified, screen-printed carbon electrode to detect Vibrio parahaemolyticus is presented.

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Electrochemical DNA Biosensor Foodborne Pathogen Detection Method Biosensors Modified Electrode Polylactic Acid Gold Nanoparticles Screen Printed Carbon Electrode Methylene Blue Redox Complex Immobilized Probe DNA Peak Current Hybridization Complementary Sequence Differential Pulse Voltammetry Guanine Bases Color Change Gold Salt Reduction Citrate Ions
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