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Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters
 

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Article DOI: 10.3791/56630-v 11:52 min February 3rd, 2018
February 3rd, 2018

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Resumo

This manuscript describes the applications of proton-selective electrodes and patch clamping methods to measure the activity of proton transport systems. These methods overcome some limitations of techniques commonly used to study proton transport activity, such as moderate sensitivity, time resolution and insufficient intracellular milieu control.

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Keywords: Electrophysiology Ion-selective Microelectrodes Sodium-hydrogen Exchanger NHE Proton Flux PH Regulation Whole Cell Patch Clamping Proton-selective Microelectrode Epithelium Ion Transport Electro-neutral Transporters
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