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成年大鼠心肌细胞的分离与培养

JoVE Journal
Medicina

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JoVE Journal Medicina

Isolation and Cultivation of Adult Rat Cardiomyocytes

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成年大鼠心肌细胞的分离与培养

DOI:

10.3791/56634-v

•

11:06 min

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October 19, 2017

•

Franziska Nippert, Rolf Schreckenberg, Klaus-Dieter Schlüter

¹Institute of Physiology,Justus Liebig University Giessen

Capítulos

00:05Título
01:15Preparation of Langendorff Perfusion System
03:09Extraction and Isolation of the Heart
08:21Results: The Appearance and Behavior of Adult Rat Ventricular Cardiomyocytes (ARVC) during Six Days of Cultivation
10:02Conclusion

Summary

Tadução automática

在这里, 我们提出了一个分离和培养成年大鼠心室心肌细胞 (ARVC) 的协议。隔离 ARVC 可用于短期和长期种植。ARVC 的分离和培养可在开发新的心脏疾病治疗方案中发挥关键作用。

Tags

Adult Rat Cardiomyocytes Cardiac Biology Cardiac Pathophysiology Cardiac Dedifferentiation Cardiac Hypertension Heart Failure Langendorff Perfusion System Collagenase Powell Medium Wistar Rats

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