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1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group,German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology,Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology,Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4),Robert Koch Institute
Capítulos
- 00:05Título
- 00:55Bacillus subtilis Spore Production and Purification and Sample Preparation of Aerosol-deposited Bacillus subtilis Spores
- 03:03Low Pressure Plasma Treatment and Recovery and Evaluation of Spore Survival
- 05:10Live Cell Microscopy, Tracking of DNA Repair Processes in Germinating Spores, and Scanning Electron Microscopy
- 07:27Results: Bacillus subtilis Spore Survival After Low Pressure Plasma Sterilization
- 09:02Conclusion
Ce protocole illustre les étapes consécutives importantes nécessaires pour évaluer la pertinence de la surveillance des paramètres de vitalité et des processus de réparation de l’ADN dans la relance des spores de Bacillus subtilis après traitement par plasma basse pression par suivi réparation de l’ADN marquées fluorescence protéines par microscopie confocale résolue dans le temps et la microscopie électronique à balayage.
