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小鼠尿的解剖和外植体培养对囊附着的作用分析

JoVE Journal
Medicina

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JoVE Journal Medicina

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

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小鼠尿的解剖和外植体培养对囊附着的作用分析

DOI:

10.3791/56712-v

•

09:30 min

•

January 13, 2018

•

Kerstin Hadamek², Angelika Keller², Antje Gohla²

¹Institute of Pharmacology and Toxicology,University of Würzburg, ²Rudolf Virchow Center for Experimental Biomedicine,University of Würzburg

Capítulos

00:05Título
00:42Coating of Microtiter Plates with α4β1 Integrin for Ex Utero Allantois Explant Culture
01:54Uterus Dissection from a Pregnent Mouse at DPC 8
03:24Embryo and Allantois Dissection and Ex Utero Allantois Cultre
06:18Staining Explanted Allantoides with Endothelial Cell Marker CD31
08:07Results: Allantois Attachment and Immunohistochemistry
08:54Conclusion

Summary

Tadução automática

我们描述了一个体外测定模型囊附着, 第一步胎盘形成。该协议展示了小鼠 allantoides 在固定化α4β1整合素上的解剖和外植体培养。尿附件是在预定的时间点显微镜下评估的。

Tags

Keywords: Murine Allantois Allantoic Attachment Placenta Formation Embryo Development In Vitro Analysis Alpha 4/beta 1 Integrin Explant Culture Dissection Uterus Mouse Embryo

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