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Zika Virus Specific Diagnostic Epitope Discovery
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Zika Virus Specific Diagnostic Epitope Discovery

Zika Virus Specific Diagnostic Epitope Discovery

DOI:
10.3791/56784-v

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11:37 min

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December 12, 2017

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, , , ,
1Department of Basic Sciences and Craniofacial Development,New York University College of Dentistry, 2Rheonix, Inc., 3Department of Medicine,New York University Langone School of Medicine

Capítulos

  • 00:05Título
  • 01:21Installing the ZIKV High-density Peptide Microarray Slide in a Specific Incubation Tray
  • 02:38Background Interation Detection: Staining the Microarray with Secondary Antibodies
  • 04:59Exposure of the Microarray to Host Serum
  • 06:08Staining with Secondary Antibodies and Labeled Control Antibodies
  • 07:12Microarray Scanning, Anaysis, and Storage
  • 09:10Results: Peptide Microarray Scanned Images and Representative Zika Virus Epitope Consensus Sequence Reacting with IgM on the Host Serum Sample
  • 10:11Conclusion

Summary

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In this protocol, we describe a technique to discover Zika virus specific diagnostic peptides using a high-density peptide microarray. This protocol can readly be adapted for other emerging infectious diseases.

Tags

Zika VirusDiagnostic AssayAntibodiesIgMIgGMicroarrayProteomeEpitopesSerumPathogensProtein SequenceIncubation TrayGlass SlideSealBlocking BufferSecondary Antibody

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