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Developmental Biology
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JoVE Journal Developmental Biology
Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation
 

Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

Article DOI: 10.3791/56835-v • 08:25 min • January 28th, 2018
January 28th, 2018 •
1, 2, 2, 1, 1, 1, 1, 2, 1
1Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 2Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Szent Istvan University

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Protocols for European eel maturation and sperm cryopreservation have been improved over the last years. This article describes the best protocol available using human chorionic gonadotropin (hCG) for inducing maturation and methanol as cryoprotectant.

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Handling Treatment Male European Eels Anguilla Anguilla Hormonal Maturation Sperm Cryopreservation Human Chorionic Gonadotropin HCG Sperm Quality Extenders Cryoprotectants Cooling And Thawing Times Tanaka's Extender Solution Methanol Toxicity Sperm Motility
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