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16S rRNA-扩增子测序法对粪便微生物特性的指导协议

JoVE Journal
Biologia

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JoVE Journal Biologia

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

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16S rRNA-扩增子测序法对粪便微生物特性的指导协议

DOI:

10.3791/56845-v

•

08:05 min

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March 19, 2018

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Ayelet Di Segni, Tzipi Braun, Marina BenShoshan, Sarit Farage Barhom, Efrat Glick Saar, Karen Cesarkas, James E. Squires, Nathan Keller³, Yael Haberman⁴

¹Sheba Medical Center, ²Children’s Hospital of Pittsburgh of UPMC, ³Ariel University, ⁴Cincinnati Children’s Hospital Medical Center

Capítulos

00:04Título
00:59Sample Handling and DNA Extraction
02:12PCR and Library Preparation
05:30Results: 16S RNA-Seq of Prepared Libraries
07:19Conclusion

Summary

Tadução automática

这篇手稿描述了一个详细的标准化协议高通量 16S rRNA 扩增子测序。该协议引入了一个集成的, 统一的, 可行的, 廉价的协议, 从粪便样本收集, 通过数据分析。该协议允许分析大量的样本, 严格的标准和几个控制。

Tags

Keywords: 16S RRNA-Amplicon Sequencing Fecal Microbiome Microbial Composition Aerobic And Anaerobic Communities High-throughput Fecal Sample Processing DNA Extraction PCR Primer Design 96-well Plate Setup Triplicate Reactions PCR Master Mix

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