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Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls
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Capítulos
- 00:05Título
- 00:51Jugular Catheter Implantation and Carotids Preparation
- 05:11Assessment of Rolling and Adhering Leukocytes/LysM+ Cells with Intravital Video Microscopy
- 07:29Results: Leukycote Rolling and Adhesion in Angiotensin II-Infused Mice
- 09:18Conclusion
This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.
