Journal
/
Genética
/
Production and Purification of Baculovirus for Gene Therapy Application
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Production and Purification of Baculovirus for Gene Therapy Application

Production and Purification of Baculovirus for Gene Therapy Application

DOI:
10.3791/57019-v

•

06:11 min

•

April 09, 2018

•

, ,
1Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, 2University of Cincinnati College of Medicine, 3Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia

Capítulos

  • 00:04Título
  • 00:55Propagation and Harvest of Baculovirus
  • 02:20Purification of Baculovirus Vector
  • 04:00Concentration of Baculovirus
  • 05:09Results: The Yield of Infectious Baculovirus During Heparin Affinity Chromatography
  • 05:42Conclusion

Summary

Tadução automática

Tadução automática

In this protocol, baculovirus is produced by transient transfection of baculovirus plasmid into Sf9 cells and amplified in a serum-free suspension culture. The supernatant is purified by heparin affinity chromatography and further concentrated by ultracentrifugation. This protocol is useful for the production and purification of baculovirus for gene therapy application.

Tags

AI-based Gene TherapyBaculovirus ProductionBaculovirus PurificationHeparin Affinity ChromatographyUltracentrifugationSf9 Cell CultureViral VectorGene TransferVaccine Development

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/57019?article_type=v

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
check_url/pt/57019?article_type=v

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
check_url/pt/57019?article_type=v

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
check_url/pt/57019?article_type=v

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
check_url/pt/57019?article_type=v

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
check_url/pt/57019?article_type=v

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
check_url/pt/57019?article_type=v

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
check_url/pt/57019?article_type=v

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
check_url/pt/57019?article_type=v

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
check_url/pt/57019?article_type=v

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Read Article