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化学共轭法在戊型肝炎病毒纳米粒子表面功能化中的应用

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Bioengenharia

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JoVE Journal Bioengenharia

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

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化学共轭法在戊型肝炎病毒纳米粒子表面功能化中的应用

DOI:

10.3791/57020-v

•

09:12 min

•

May 11, 2018

•

Chun Chieh Chen*¹, Marie Stark*¹, Mo Baikoghli, R. Holland Cheng

¹Department of Molecular and Cellular Biology,University of California Davis

Capítulos

00:04Título
00:50HEVNP Characterization
02:46Chemical Conjugation of HEVNPs with Biotin, Cancer Targeting Ligand, and Fluorophores
07:05Results: Production of LXY30-HEVNPs and Binding/Internalization of Fluorescently Labeled LXY30-HEVNPs in MDA-MB-231 Breast Cancer Cells
08:35Conclusion

Summary

Tadução automática

我们已将戊型肝炎病毒的衣壳蛋白作为 theranostic 纳米微粒 (HEVNP) 进行了设计。HEVNP 自组装成一个稳定的 icosahedral 笼在黏膜分娩。在这里, 我们描述了 HEVNPs 的改变的肿瘤靶向性的变异表面暴露残留到半胱氨酸, 共轭合成配体, 具体绑定肿瘤细胞。

Tags

Keywords: HEV Nanoparticles Surface Functionalization Chemical Conjugation Targeting Diagnosis Treatment Nano Therapeutics TEM Imaging Maleimide-linked Biotin Dialysis

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