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JoVE Journal Neuroscience
Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue
 

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

Article DOI: 10.3791/57123-v • 06:16 min • May 8th, 2018
May 8th, 2018 •
1,2,3, 4, 1,3, 1,3, 1,2,3
1Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of Medicine, 2Brain Korea 21 PLUS Project for Medical Science, Yonsei University, 3The Spine and Spinal Cord Institute, Biomedical Center, Gangnam Severance Hospital, Yonsei University College of Medicine, 4Columbia University College of Physicians and Surgeons

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Here, we present two novel methodologies, psPACT and mPACT, for achieving maximal optical transparency and subsequent microscopic analysis of tissue vasculature in the intact rodent whole CNS.

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Passive Clearing Methods Rapid Production Optical Transparency Whole CNS Tissue PsPACT MPACT Electrophoretic Tissue Clearing System Human Disease Research Model Alzheimer's Disease Injury And Degeneration Tumorigenesis Mouse CNS Tissue Modified Passive Clearing PACT Sample Preparation PACT Cocktail Solution VA-044 Powder 4%PFA 4%acrylamide Perfusing Tissues 50-milliliter Tube Chilled PACT Solution Four Degrees Celsius 24 Hours Tissue Gel Hybridization System Nitrogen Tank
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