Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

April Maschmann; Cody Masters; Melissa Davison; Joshua Lallman; Drew Thompson; Kristy L. Kounovsky-Shafer

doi:10.3791/57141

Journal

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Bioquímica

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Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

DOI:

10.3791/57141-v

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06:58 min

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February 02, 2018

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April Maschmann, Cody Masters, Melissa Davison, Joshua Lallman, Drew Thompson, Kristy L. Kounovsky-Shafer

¹Department of Chemistry,University of Nebraska – Kearney

Capítulos

00:05Título
00:42Preparation of Dyes, Buffers, and Agarose Gel
01:34Preparation of Stained DNA and Restriction Enzyme Assay
02:47Electroeluting Stained DNA in an Agarose Gel
04:44Results: Digestion of Cyanine Dye-treated Lambda DNA
06:08Conclusion

Summary

Tadução automática

Staining DNA molecules for fluorescence microscopy allows a scientist to view them during an experiment. In the method presented here, DNA molecules are pre-stained with fluorescent dyes and digested with methylation and non-methylation sensitive restriction enzymes.

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

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