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革兰氏阴性菌的基本金属摄取: x 射线荧光、放射性同位素和细胞分馏
JoVE Journal
Química
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JoVE Journal Química
Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
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革兰氏阴性菌的基本金属摄取: x 射线荧光、放射性同位素和细胞分馏

DOI:
10.3791/57169-v

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10:34 min

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February 01, 2018

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, , , , ,
1Graduate Biomedical Sciences Microbiology Theme,University of Alabama at Birmingham, 2Department of Radiology,University of Alabama at Birmingham, 3Office of the Provost,University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology,University of Alabama at Birmingham

Capítulos

  • 00:04Título
  • 01:09Cell Fractionation and Protein Crystallization
  • 03:52X-ray Fluorescence (Using GM/CA Beamline Software)
  • 04:44MAD Scan for Metal Energy Absorption Peak (Using GM/CA Beamline Software)
  • 05:45Analytical Protocol for Radioactive Metal Uptake Assay
  • 07:24Results: Extraction of Bacterial Periplasm from Cytoplasm in Gram-negative Bacteria
  • 09:14Conclusion

Summary

Tadução automática

Tadução automática

本文提出了一种周过渡金属伴侣在其本机结合的背景下提取的协议, 并给出了 X 射线荧光和 radiometal 吸收对其基质含量的生物物理表征。

Tags

Metal UptakeGram-negative BacteriaX-ray FluorescenceRadioisotopesCell FractionationABC Transport SystemsSubstrate BindingPeriplasmOsmotic ShockSpheroplastsProtein Extraction

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