Journal
/
Imunologia e Infecção
/
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

DOI:
10.3791/57196-v

•

08:52 min

•

March 23, 2018

•

, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Capítulos

  • 00:04Título
  • 00:49Growth of Pseudomonas aeruginosa Colony Biofilms and Overlaying Colonies with Agar
  • 02:33Fixation and Sample Processing
  • 03:02Sample Embedding and Sectioning
  • 05:23Heat Fixing, Rehydration, and Mounting
  • 06:48Results: Microscopic Analysis of Microbial Colony Biofilms
  • 08:01Conclusion

Summary

Tadução automática

Tadução automática

We describe fixation, paraffin embedding, and thin sectioning techniques for microbial colony biofilms. In prepared samples, biofilm substructure and reporter expression patterns can be visualized by microscopy.

Tags

Paraffin EmbeddingThin SectioningMicrobial Colony BiofilmsMicroscopic AnalysisGene ExpressionFluorescent ProteinBiofilm ArchitectureExopolymeric SubstancesAgar Tryptone SolutionP. AeruginosaColony BiofilmsEmbedding CassetteFixativePBSMicroscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/57196?article_type=v

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
check_url/pt/57196?article_type=v

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
check_url/pt/57196?article_type=v

Growth of Mycobacterium tuberculosis Biofilms
check_url/pt/57196?article_type=v

Isolation and Genome Analysis of Single Virions using ‘Single Virus Genomics’
check_url/pt/57196?article_type=v

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
check_url/pt/57196?article_type=v

Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
check_url/pt/57196?article_type=v

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
check_url/pt/57196?article_type=v

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
check_url/pt/57196?article_type=v

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
check_url/pt/57196?article_type=v

Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms

Read Article