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Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems
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Capítulos
- 00:05Título
- 00:30Adsorbing Samples to the Carbon Substrate
- 01:07Staining Using the Side Blot Method
- 02:25Staining Using the Flicking Method
- 03:24Staining Using the Rapid Flushing Method
- 04:12Results: The Ideal Negative Stain for EM is Sample Dependent
- 05:36Conclusion
Negative stain EM is a powerful technique for visualizing macromolecular structure, but different staining techniques can produce varying results in a sample dependent manner. Here several negative staining approaches are described in detail to provide an initial workflow for tackling the visualization of challenging systems.
