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世代と複雑な染色体と細胞周期逮捕細胞の分離
JoVE Journal
Pesquisa do Câncer
This content is Free Access.
JoVE Journal Pesquisa do Câncer
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
DOI:

05:22 min

April 13, 2018

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Capítulos

  • 00:04Título
  • 00:32Synchronization of RPE-1 hTERT Cells
  • 01:34Generation of Aneuploid Cells by Interference with the Activity of the Mitotic Kinase Mps1 and Removal of Aneuploid Cycling Cells to Enrich the ArCK Population
  • 03:36Results: In Vitro Isolation and Characterization of Aneuploid Cells with Complex Karyotypes (ArCK)
  • 04:35Conclusion

Summary

Tadução automática

異数性は、ゲノムの不安定性、複雑な染色体を持つ細胞を細胞周期逮捕を最終的に生成に します。このペーパーでは、複雑な染色体を分割するために停止を構成する細胞を分離する簡単で便利な方法を提供します。

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