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Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
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JoVE Journal Bioengenharia
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

DOI:
10.3791/57242-v

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07:05 min

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April 04, 2018

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, , , , , , , , ,
1Department of Micro Engineering,Kyoto University, 2Graduate School of Medical Sciences,Kyushu University, 3International Research Center for Medical Sciences (IRCMS),Kumamoto University

Capítulos

  • 00:04Título
  • 00:48Polydimethylsiloxane (PDMS) Layer Fabrication and Assembly
  • 01:48Microfluidic Device Cell Seeding
  • 05:12Results: Representative Vascular Lumen Formation
  • 06:42Conclusion

Summary

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Tadução automática

The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.

Tags

Microfluidic DevicePerfusable Vascular NetworkSpheroidHLFRFP-HUVECGFP-HUVECEndothelial MediumMaster Mix SolutionRegenerative Medicine

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